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(Peer Review) HERITABILITIES OF BODY SIZE BY GROWTH HORMONE (GH-Msp1)GENOTYPES USING PCR-RFLP IN ONGOLE GRADE CATTLE

Paputungan, Umar (2015) (Peer Review) HERITABILITIES OF BODY SIZE BY GROWTH HORMONE (GH-Msp1)GENOTYPES USING PCR-RFLP IN ONGOLE GRADE CATTLE. Faculty of Animal Agriculture, UNDIP.

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      Abstract

      ABSTRAK Tampilan genetik dalam istilah heritabilitas yang merupakan faktor penting pada sifat ekonomis ternak untuk pewarisan ukuran tubuh belum sepenuhnya dikaji pada ternak sapi Peranakan Ongole (PO). Tujuan penelitian ini adalah menentukan nilai-nilai heritabilitas bobot badan, lingkar dada dan panjang badan pada sapi PO. Total 37 sampel darah diambil dari tetua induk betina dan 2 sampel darah dari tetua pejantan sapi Ongole. Semua sampel darah diuji gambaran keberadaan genotipe lokus hormon pertumbuhan (GH) menggunakan metode PCR-RFLP yang melibatkan enzim Msp1 pada “agarose-gel” (1,2%) melalui elektroforesis. Data dianalisis menggunakan program stastistik dalam Excel XP. Hasil analisis memperlihatkan bahwa rata-rata estimasi fenotipe populasi sapi PO untuk bobot hidup, lingkar dada dan panjang badan masing-masing adalah 445,41 ± 45,95 kg; 175,35 ± 4,11 cm dan 139,70 ± 5,73cm. Nilai dugaan heritabilitas bobot badan, lingkar dada dan panjang badan masing-masing adalah 0,24; 0,003 and 0,41. Nilai heritabilitas ternak untuk bobot badan dan panjang badan dapat dikategorikan ke dalam nilai tampilan genetik sedang (moderate) sampai tinggi (high), sedangkan nilai heritabilitas lingkar dada dapat dimasukkan dalam kategori haritabilitas rendah (low) untuk sifat ekonomis ternak. Kata Kunci: Enzim GH-Msp1, sapi Peranakan Ongole, sifat genotipe, ukuran tubuh ABSTRACT Genotypic performance in term of heritability as the crucial factor of animal economical traits for body size inheritance had not been fully studied in Ongole-grade cattle. The objectives of this research were to define the heritability values of live weight, chest girth and body length in Ongole-crossbred cattle. Total of 37 blood samples were collected from parental cows and 2 blood samples from parental Ongole breed bulls. All blood samples were screened for the presence of growth hormone (GH) locus using PCR-RFLP method involving restricted enzyme Msp1 on agarose-gel (1.2%). Data were analyzed using statistical program in Excel XP. Results showed that the phenotypic estimation average of Ongole grade cattle population of live weight, chest girth and body length were 445.41 ± 45.95 kg, 175.35 ± 4.11 cm, and 139.70 ± 5.73cm, respectively. The heritability values of animal live weight, chest girth and body length in this study were 0.24, 0.003, and 0.41, respectively. These heritability values of animal live weight and body length would be categorized as moderate to high genotypic performance values, while the heritability of animal chest girth was included in low heritability standard of the animal economical trait performance. Keywords: GH-Msp1 enzyme, Ongole-grade cattle, genotypic traits, body size

      Item Type: Other
      Subjects: S Agriculture > SF Animal culture
      Divisions: Fakultas Peternakan > lmu Peternakan
      Depositing User: Mr. Benhard W. Tampangela, ST
      Date Deposited: 24 Feb 2016 12:44
      Last Modified: 24 Feb 2016 12:44
      URI: http://repo.unsrat.ac.id/id/eprint/1060

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