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DNA Barcoding Analysis of matK Gene of Some Syzygium Species

Tallei, Trina Ekawati (2016) DNA Barcoding Analysis of matK Gene of Some Syzygium Species. In: BIOINFORMATICS WORKSHOP 2016: Developing knowledge and skill in bioinformatics for Young Indonesian Scientists in improving research quality in life science and sustainable exploration of biodiversity in Indonesia, 13 – 15 September 2016, Al Azhar University Jakarta.

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Syzygium is a genus of flowering plants that belongs to the myrtle family (Myrtaceae). Accurate identification of Syzygium species using traditional methods can take a long time due to lack of knowledge about plants and/or lack of flowers and fruit characters required for identification. Reliability or accuracy of identification relies on expert determination, which is not always readily available. Moreover, Syzygium genera exhibit many overlapping characters, making them difficult to identify. In this research, the utility of matK gene as DNA barcoding in Syzygium was assessed. The assessment included barcoding gap, intraspecific and interspecific divergence, as well as phylogenetic tree reconstruction. The evolutionary history was inferred by using UPGMA method. Estimation of evolutionary divergence between sequence was analysed using Kimura’s two-parameter. Evolutionary analyses were conducted in MEGA7.0.18 The result showed an overlapping distribution of interspecific divergence and intraspecific distance for matK region. The phylogeny indicated that some of Syzygium cumini are embeded within other Syzygium species. Therefore, matK gene is not suitable to be used as barcode region for identification of Syzygium species.

Item Type: Conference or Workshop Item (Poster)
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Fakultas Matematika dan IPA > Biologi
Depositing User: Mr. Benhard W. Tampangela, ST
Date Deposited: 19 Oct 2016 06:54
Last Modified: 01 Dec 2016 02:26
URI: http://repo.unsrat.ac.id/id/eprint/1258

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