Dien, Grace Sisilia and Manueke, Jusuf and Salaki, Ch. L.
(2013)
EKSPLORASI BAKTERI Bacillus sphaericus ISOLAT TANAH VEGETASI
TUMBUHAN HUTAN ALAM GUNUNG MASARANG DI TOMOHON
SEBAGAI AGEN PENGENDALI HAYATI LARVA NYAMUK Aedes aegypti.
Eugenia , 19 (2).
pp. 120-127.
ISSN 0854-0276
Abstract
The world was undertaken with the aim to obtain isolates of Bacillus sphaericus from soil samples of natural forest vegetation in Mount Masarang, T omohon. This research was carried out by several phases: 1) Soil sampling, 2) Isolation of 8. sphaericus from soil samples, 3) Insect colonization, or multiplication test, 4) Screening, or test the power to kill isolates 8. sphaericus against Aedes aegypti larvae, and 5) Selection of potential isolates based on killing power values. This research was
conducted at the Laboratory of Microbiology and Plant Pathology, Department of Plant Pests and
Diseases, starting from soil sampling of natural forest land in Mount Masarang then continued isolation
and maintenance stages of mosquito larvae, in March-November 2013. The results showed that, the
entomopathogenic bacteria exploration from the natural forests of Mount Masarang successfully
obtained 51 isolates were identified as members of the 8. sphaericus. The results of testing 51 isolates
toward the power to kill the larvae of Ae. aegypti, only 4 (four) isolates were considered to be potentially
effective in causing mortality > 50% of test larvae after 24 hours treatment. Mortality percentage of
Ae. aegypti was found on the highest concentration of 107 on the Cempaka soil 75%, followed by Pakis
soil, Nantu soil and Beringin soil 70%, 60% and 55%, respectively.
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