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EKSPLORASI BAKTERI Bacillus sphaericus ISOLAT TANAH VEGETASI TUMBUHAN HUTAN ALAM GUNUNG MASARANG DI TOMOHON SEBAGAI AGEN PENGENDALI HAYATI LARVA NYAMUK Aedes aegypti

Dien, Grace Sisilia and Manueke, Jusuf and Salaki, Ch. L. (2013) EKSPLORASI BAKTERI Bacillus sphaericus ISOLAT TANAH VEGETASI TUMBUHAN HUTAN ALAM GUNUNG MASARANG DI TOMOHON SEBAGAI AGEN PENGENDALI HAYATI LARVA NYAMUK Aedes aegypti. Eugenia , 19 (2). pp. 120-127. ISSN 0854-0276

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      Abstract

      The world was undertaken with the aim to obtain isolates of Bacillus sphaericus from soil samples of natural forest vegetation in Mount Masarang, T omohon. This research was carried out by several phases: 1) Soil sampling, 2) Isolation of 8. sphaericus from soil samples, 3) Insect colonization, or multiplication test, 4) Screening, or test the power to kill isolates 8. sphaericus against Aedes aegypti larvae, and 5) Selection of potential isolates based on killing power values. This research was conducted at the Laboratory of Microbiology and Plant Pathology, Department of Plant Pests and Diseases, starting from soil sampling of natural forest land in Mount Masarang then continued isolation and maintenance stages of mosquito larvae, in March-November 2013. The results showed that, the entomopathogenic bacteria exploration from the natural forests of Mount Masarang successfully obtained 51 isolates were identified as members of the 8. sphaericus. The results of testing 51 isolates toward the power to kill the larvae of Ae. aegypti, only 4 (four) isolates were considered to be potentially effective in causing mortality > 50% of test larvae after 24 hours treatment. Mortality percentage of Ae. aegypti was found on the highest concentration of 107 on the Cempaka soil 75%, followed by Pakis soil, Nantu soil and Beringin soil 70%, 60% and 55%, respectively.

      Item Type: Article
      Subjects: S Agriculture > S Agriculture (General)
      Divisions: Fakultas Pertanian > Agroekoteknologi
      Depositing User: Mr. Benhard W. Tampangela, ST
      Date Deposited: 07 Feb 2018 15:13
      Last Modified: 24 Nov 2020 11:57
      URI: http://repo.unsrat.ac.id/id/eprint/1886

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