Rondonuwu, Frans B. and Paath, Johanna M. and Manengkey, Guntur S. J. and Montong, Vivi B. and Pinaria, Arthur and Assa, Berty H. and Sembel, Dantje T. and Liew, Edward C. Y.
(2008)
DIAGNOSIS PENYAKIT BUSUK BATANG PANILI (Vanilla planifolia Andrews) DI MINAHASA.
Eugenia , 14 (3).
pp. 290-299.
ISSN 0854-0276
Abstract
The objectives of this research was to know the causing of this disease and their infection mode.
Disease diagnosis followed Koch’s postulate, namely: (1) analysis of disease symptom varieties, (2) isolation,
purification, and identification, (3) pathogenicity tests, and (4) reisolation. Pathogenicity tests to F. oxysporum
and Colletotrichum gloeosporioides by using spraying method of spore suspension, to Fusarium oxysporum,
Fusarium solani, Fusarium subglutinans, and C. gloeosporioides by using soil inoculation, and to Rhizoctonia sp
and C. gloeosporioides by using inoculum sticking method. All of pathogenicity tests were arranged according to
completely randomized design.
The fungi which were associated with a diseased vanilla stem tissue, namely: Colletotrichum
gloeosporioides, Rhizoctonia sp., Fusarium oxysporum, Fusarium solani,Fusarium subglutinans, Fusarium
semitectum, Fusarium pseudograminearum, dan Fusarium proliferatum. Among these fungi only F. oxysporum
and C. gloeosporioides as the cause of vanilla stem rot disease. The mode of infection of F. oxysporum only
through wounds, and isolates from difference location have shown difference virulence. The inoculum of C.
gloeosporioides only infect through wounds.
Keywords: Colletotrichum gloeosporioides, Rhizoctonia sp., Fusarium oxysporum, Fusarium
solani, Fusarium subglutinans, Fusarium semitectum, Fusarium
pseudograminearum, Fusarium proliferatum, Endophyte, Koch’s postulate
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