Dingse, Pandiangan and Dennie Heroike, Rompas and Aritonang, Henry Fonda and Rizkita Rachmi, Esyanti and Erly, Mawarni
(2006)
Pengaruh Triptofan pada Pertumbuhan dan Kandungan Katarantin
dari Kalus Catharanthus roseus.
Jurnal Matematika dan Sains, 11 (4).
pp. 111-115.
ISSN 0854-5154
Abstract
Pengaruh triptofon terlmdap pefiumbulnn dan kandungatT kcLtaratltitt pada kttLtur kalus Catharanthus roselis telah
diteliti- Pertuntbuhan kalus C. roseus-],arlg, tliberitriptofan 100, 125 dan225 mg/Lrendahpada hari ke-21, trLmurtyrutg
diberi penantbahan 150, 175 datt 200 ng/L pertunbuhannya lebih baikctari kontrol. Kcutclulgan katarantirt kontrol
ataLt tatTpa triptofan adalah 123,22 pg/g bk. Kandungan katarantitt pada keencun kalus yang diberi perlakuan triptofan
100' 125, 150, 175,200,225 mg/L adalah 485,00, 588,32,875,10, 905,26,781,10 dan 950,i4 pg/g bk. pertakuail,ang
optimal untuk pefiumbuhan dan produksi katarantin dengan pencntbahan prekursor triptofan aiatah 175 mg/L a"",rgol
peningkatan kandungan katarantin sebesar I 13,90 7o .
Research on the growtlt and catharctnthine content of Catharanthus roseus callus w,lticlt were treatel with tryptophan
had been done. Wet and dry weight o/Catharanthus roseus collus clecreased wlten treated with 100, 125 and 2-25 nry/L
tr1|ptopltan' but callus treated with 150, 175 cutd 200 ntg/L tryptophan grew better. Catharanthine contents ofihe
kontrol callus was 423,22 1tg/g dw. The treatrnettt ccLlli containi +'ss,oo,-5gg,32, g75,10, g05,26, 7g4,10 artd 950,54
1tg/g dw of catlmrantltine respectively. The optinml tryptophan treattilett for callus growth ancl cathara,tltirrc
production was 175 mg/L tryptopltcut with a I 13,90 To. increase of catharanthine content.
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