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POLA KANDUNGAN KATARANTIN AGREGAT SEL Chatharanthus roseus DALAM ERLENMEYER

Dingse, Pandiangan (2010) POLA KANDUNGAN KATARANTIN AGREGAT SEL Chatharanthus roseus DALAM ERLENMEYER. Eugenia , 16 (3). pp. 216-226. ISSN 0854-0276

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    Abstract

    Bioactive compound content in vitro culture can be improved by feeding precursor, The effect of tryptophan on growth and catharanthine production in cell aggregates of Catharanthus roseus (1.) G Don as well as in medium has been studied Agregate cells of C. roseus were cultured in Murashige and Skoog (MS) medium supplemented with 2 mg/L NAA and 0,2 mg/L kinetin. Agregate cells culture were divided into control and treated groups. The treated group u/as fed by 150 mg/L tryptophan while the control was aciciecj by ciestilied water. The growth of cells and catharanthine production in the cell aggregates as well as rn medium were observed on day 0, 4,7, 10. 14, and 21 Qualitative and quantitative analysis of catharanthine were carried out using High Performance Liquid Chromatography (HPLC). The resu[ showed that the grcMh pattern in the contrcl and treated cell aggregates were similar, except additioned oi tryptophan coulci increase the growih of the cell aggregates, producing longer growth phase. The addition of 150 mg/L tryptophan also increased catharanthin production. The highest concentration of catharanthine was produced in cellaggregales2261,6Bt17,05 pg/g DW on the 14rh days Keywo rd s

    Item Type: Article
    Uncontrolled Keywords: Katarantin, Triptofan, Catharanthus roseus, agregat sel
    Subjects: Q Science > Q Science (General)
    Divisions: Fakultas Matematika dan IPA > Biologi
    Depositing User: Steven Ch. Kaunang
    Date Deposited: 03 Mar 2014 11:26
    Last Modified: 03 Mar 2014 11:26
    URI: http://repo.unsrat.ac.id/id/eprint/417

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