Phylogenetic analysis of partial CAO gene of Prochloron didemni isolated from Lissoclinum patella of Manado bay, North Sulawesi.

Wullur, Stenly (2013) Phylogenetic analysis of partial CAO gene of Prochloron didemni isolated from Lissoclinum patella of Manado bay, North Sulawesi. Seminar Tahunan ke-2 dan Workshop Bioteknologi Kelautan Perikanan. Manado, 16-18 September 2013.

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Abstract

Prochloron spp. are obligate photosynthetic cyanobacterial symbionts found in cellulose tunics of many didemnid family ascidians. This specific bacterium has a housekeeping gene called cao (chlorophyll b synthase / chlorophyll a oxygenase) that is involved in chlorophyll b biosynthesis. Chlorophyll b is synthesized from chlorophyll a by chlorophyll a oxygenase, which contains consensus sequences for a (2Fe-2S) Rieske center and for mononuclear iron binding sites. Our investigation shows that Prochloron spp. isolated from Lissoclinum patella of Manado Bay is a Prochloron didemni as this species showed 100% identity to Prochloron didemni in a Blast search program in NCBI database. The partial Cao gene used for identification contains the conserved Rieske domain with 100% similarity to Prochloron ididemni. Moreover, phylogentic relationship of these organisms inferred from Cao amino-acid sequence indicates the bootstrap values of 100 in the inner nodes of both species indicating that theses two organisms are identical

Item Type:	Article
Subjects:	Q Science > Q Science (General)
Divisions:	Fakultas Perikanan dan Ilmu Kelautan > lmu Kelautan
Depositing User:	Mr. Benhard W. Tampangela, ST
Date Deposited:	30 Jul 2015 07:18
Last Modified:	30 Jul 2015 07:18
URI:	http://repo.unsrat.ac.id/id/eprint/848

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