Rizkita Rachmi, Esyanti and Dingse, Pandiangan and Veny, usviani
(2006)
Enhancement of Catharanthine Production in Catharanthus roseus Cell Cultures by Adding Tryptophan as Precursors.
In: International Conference on Mathematics and Natural Science ICMNS, November 29-30, 2006, Bandung-Indonesia.
Abstract
Feeding precursor has a role on the secondary metabolite enhancement in in vitro culture. The effect of tryptophan on catharanthine production was studied in aggregate cells of Catharanthus roseus (L.) G Don. Aggregate cells of C. roseus were cultured in Murashige and Skoog (MS) medium supplemented with 2 ppm NAA and 0.ppm kinetin. Aggregate cells culture were devided into control and treatment groups and were fed by 100, 125, 150, 175, 200, 225 mg/L tryptophan, respectively. The growth of cells and the catharanthine production in the aggregate cells as well as in medium were observed on the 1st and 10th days. Qualitative and quantitative analysis were conducted using High Performance Liquid Chromatography (HPLC) in VP-ODS C-18 column, eluted using methanol : acetonitrile : diamonium hydrogen phosphate (3 : 4 : 3), rate 1 ml/minute. The results showed that catharanthine was detected in the aggregate cells and culture medium. The highest concentration of catharanthine in the aggregate cell and the medium were obtained on the addition of 175 mg/L tryptophan, i.e. 1650.48 ug/g and 10329.75 ug/L respectively. Tryptophan feeding enhanced catharanthine content in aggregate cell by 171.02%, as well as its secretion into medium by 329.34%.
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