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The Effect Tryptophan Feof eding on Growth, Protein Content and TDC activity Catharanthus roseus (L) G.Don Cell

Pandiangan, Dingse and Tilaar, Wenny and Karyono, . and Esyantic, Rizkita Rahmi and Subarnas, Anas (2010) The Effect Tryptophan Feof eding on Growth, Protein Content and TDC activity Catharanthus roseus (L) G.Don Cell. In: Third International Conference on Mathematics and Natural Sciences (ICMNS 2010).

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      Abstract

      There are various ways to increase secondary metabolite content in tissue culture, one of them is precursor feeding. Therefore research to study of the growth, protein content, and TDC activity of Catharanthus roseus (L) G. Don aggregate cell culture with the addition of tryptophan as a precursor has been conducted. The aim of the research were to evaluate the growth, protein content, and TDC activity that correlated to enhancement of catharanthine content. The research was carried out experimentally in the laboratory. The concentrations of tryptophan precursors used were 50-250 mg/L for the culture of cell aggregates. Growth was determined by measuring changes of weight and observing the morphological changes. Protein content was determined by the methods of spectrophotometry. TDC activity was determined by the methods of spectrofluorometry. The maximum growth of cell aggregate culture occurred in the treatment of tryptophan 150 mg/L in airlift bioreactor. The addition of tryptophan induced protein synthesis and TDC activity in aggregate cell culture of C.roseus. The highest of protein content and TDC activity occurred in the treatment of 250 mg/L after 10days of culture . The highest TDC activity of 3.459±0.001 units after 10 days of culture. The tryptophan treatment enhanced protein content and TDC activity. The results concluded that the treatment of tryptophan as a precursor increased protein content and TDC activity. The optimum growth on tryptophan concentration 150 mg/L after 10 days of culture.

      Item Type: Conference or Workshop Item (Paper)
      Subjects: Q Science > Q Science (General)
      Q Science > QD Chemistry
      S Agriculture > S Agriculture (General)
      S Agriculture > SB Plant culture
      Divisions: Fakultas Pertanian > Agroekoteknologi
      Depositing User: Mr. Benhard W. Tampangela, ST
      Date Deposited: 20 May 2016 10:26
      Last Modified: 30 Jun 2016 11:19
      URI: http://repo.unsrat.ac.id/id/eprint/1115

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